FIGURE

Fig. 6

ID

ZDB-FIG-160404-33

Publication

Houbrechts et al., 2016 - Deiodinase knockdown affects zebrafish eye development at the level of gene expression, morphology and function

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Fig. 6

Effects of deiodinase KD on relative rod and cone photoreceptor number. (A-L) Immunohistochemical stainings with Zpr3, Zpr1, anti-blue opsin and anti-UV opsin antibodies on cryosections of the eye of control (A,D,G,J), D1D2MO (B,E,H,K) and D3bMO (C,F,I,L) larvae at 3 dpf. Zpr1 is a marker for both green and red cone photoreceptors, Zpr3 is a marker for rod photoreceptors and anti-blue and anti-UV opsin label the outer segment of blue and UV cone photoreceptors respectively. Blue: DAPI staining of the nuclei; Green: Zpr1/Zpr3+Alexa488 or anti-blue/anti-UV + FITC. (M-P) Data for relative rod/cone number (expressed per retinal surface area) are shown as mean + SEM (n ≥ 5 per group). Groups with no common letter are significantly different (p < 0.05). Data in (N,O,P) followed Gaussian distribution and were analyzed by one-way ANOVA with Tukey post hoc test. Data in (M) did not assume Gaussian distribution and were analyzed by Kruskal-Wallis and Dunn′s post hoc test. SCMO: control group, D1D2MO: KD of D1+D2; D3bMO: KD of D3b.

Expression Data

Genes:	arr3a opn1sw1 opn1sw2
Antibodies:	Ab1-opn1sw1 Ab1-opn1sw2 zpr-1 zpr-3
Fish:	WIK WIK + MO1-dio3b WIK + MO1-dio1 + MO1-dio2
Knockdown Reagents:	MO1-dio1 MO1-dio2 MO1-dio3b
Anatomical Terms:	blue sensitive photoreceptor cell photoreceptor outer segment retinal cone cell retinal photoreceptor layer blue sensitive photoreceptor cell retinal photoreceptor layer green sensitive photoreceptor cell retinal photoreceptor layer red sensitive photoreceptor cell retinal photoreceptor layer UV sensitive photoreceptor cell retinal rod cell UV sensitive photoreceptor cell photoreceptor outer segment
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WIK + MO1-dio3b WIK + MO1-dio1 + MO1-dio2
Knockdown Reagents:	MO1-dio1 MO1-dio2 MO1-dio3b
Observed In:	retinal cone cell retinal photoreceptor layer retinal photoreceptor layer blue sensitive photoreceptor cell retinal photoreceptor layer green sensitive photoreceptor cell retinal photoreceptor layer red sensitive photoreceptor cell retinal photoreceptor layer UV sensitive photoreceptor cell retinal rod cell
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Molecular and Cellular Endocrinology, 424, Houbrechts, A.M., Vergauwen, L., Bagci, E., Van Houcke, J., Heijlen, M., Kulemeka, B., Hyde, D.R., Knapen, D., Darras, V.M., Deiodinase knockdown affects zebrafish eye development at the level of gene expression, morphology and function, 81-93, Copyright (2016) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.