FIGURE

Fig. S6

ID

ZDB-FIG-160316-32

Publication

Xu et al., 2016 - Four and a Half LIM Domains 1b (Fhl1b) Is Essential for Regulating the Liver versus Pancreas Fate Decision and for β-Cell Regeneration

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Fig. S6

fhl1b mRNA injection partially rescues the effect of fhl1b MO knockdown.

(A-C′′) The developmental defects of the liver (A-C and white dotted circles in A′′-C′′) and β-cell formation (A-C and A′-C′) in fhl1b morphants (B-B′′) could be partially rescued by injection of fhl1b-P2A-mcherry mRNA (C-C′′), restoring liver size and the β-cell population to a degree comparable to that of control embryos (A-A′′) at 55 hpf. Fhl1b translation was monitored by mCherry expression as shown in C. (D) Quantification of the results in A-C′′. The embryos were scored as having a “reduced” or “increased” expression domain when the expression area of Tg(ins:dsRed)^m1018 and Tg(sox17:GFP)^s870 was distinctly (> 25%) smaller or larger than that of the control embryos based upon the calculation using ImageJ. A-C, bright-field images combined with fluorescent image of Tg(ins:dsRed)^m1018 and Tg(sox17:GFP)^s870 expression. A′-C′′, fluorescent images of Tg(ins:dsRed)^m1018 and Tg(sox17:GFP)^s870 expression. Dorsal views, anterior to the left. Scale bar, 20 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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