ZFIN Image: Xu et al., 2016, Fig. S6

Image

Figure Caption

Fig. S6

fhl1b mRNA injection partially rescues the effect of fhl1b MO knockdown.

(A-C′′) The developmental defects of the liver (A-C and white dotted circles in A′′-C′′) and β-cell formation (A-C and A′-C′) in fhl1b morphants (B-B′′) could be partially rescued by injection of fhl1b-P2A-mcherry mRNA (C-C′′), restoring liver size and the β-cell population to a degree comparable to that of control embryos (A-A′′) at 55 hpf. Fhl1b translation was monitored by mCherry expression as shown in C. (D) Quantification of the results in A-C′′. The embryos were scored as having a “reduced” or “increased” expression domain when the expression area of Tg(ins:dsRed)^m1018 and Tg(sox17:GFP)^s870 was distinctly (> 25%) smaller or larger than that of the control embryos based upon the calculation using ImageJ. A-C, bright-field images combined with fluorescent image of Tg(ins:dsRed)^m1018 and Tg(sox17:GFP)^s870 expression. A′-C′′, fluorescent images of Tg(ins:dsRed)^m1018 and Tg(sox17:GFP)^s870 expression. Dorsal views, anterior to the left. Scale bar, 20 µm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.