FIGURE

Fig. 4

ID

ZDB-FIG-160310-15

Publication

Chen et al., 2016 - GADD45B mediates podocyte injury in zebrafish by activating the ROS-GADD45B-p38 pathway

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Fig. 4

gadd45ba and gadd45bb overexpression in podocytes aggravates MTZ-induced proteinuria. (a) Representative fluorescence figures showing disruption of the glomerular filtration barrier by MTZ leads to accumulation of GFP fluorescence in the proximal tubules (arrowheads) in Tg(pod:Gal4;UAS-NTR-mCherry; lfabp:VDBP-GFP). mCherry fluorescence (arrows) is reduced in glomeruli and accumulated in proximal tubules presumably due to podocytes loss. (b) Quantitation of GFP by ELISA showing zebrafish expressing gadd45ba/b in podocytes exhibit more poteinuria induced following MTZ-induced podocyte injury. Results are represented as mean±S.D. (n=3), *P<0.01 versus NTR group; Δ P<0.01 versus NTR and gadd45bb group

Expression Data

Genes:	EGFP mCherry
Fish:	mi1000Tg; mi1004Tg; rw0144Tg
Condition:	chemical treatment: metronidazole
Anatomical Terms:	pronephric glomerulus pronephric tubule
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mi1000Tg; mi1004Tg; rw0144Tg mi1000Tg; mi1004Tg; mi1005Tg; rw0144Tg mi1000Tg; mi1004Tg; mi1006Tg; rw0144Tg
Condition:	chemical treatment: metronidazole
Observed In:	glomerular filtration pronephric glomerulus pronephric tubule
Stage:	Day 5

Phenotype Detail

Acknowledgments

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