Fig. S3

Loss of nr2f1b results in pericardial edema, absent parachordal vessels, subintestinal vessels (SIV) mispattern and circulation defects. (A,B) nr2f1bMO injected embryos showed severe pericardial edema at 72hpf compared to wt, in addition, there is an obvious smaller eyes in nr2f1b morphants. Quantitatively, 60% of nr2f1b morphants (n=43) with mild to severe pericardial edema compared to wt (n=49) as shown in (G). (C-F, C′-F′) nr2f1b^i1e2 MO was injected into transgenic Tg(fli:eGFP^y1;gata1:dsRed^sd2) embryos with GFP-labeled endothelial cells (C,D,E,F) and dsRed-labeled blood cells(C′,D′,E′,F′). nr2f1b morphants show mispatterned ISV, DLAV and SIV (arrow), and absent parachordal vessels (arrowhead) (C and D) and circulation defect at the ISV and DLAV and slow to lose axial circulation of the aorta and vein (D′) in the trunk region at 48hpf as compared to wild-type (C′). Vasculature and circulation defects are also observed in the head region of nr2f1b morphants (F and F′) as compared to wt (E and E′). Quantification of the number of embryos exhibiting trunk vessel circulation defects in wt (n=8) and nr2f1b morphants (n=25) as shown in (H). The scale bars in C-F and C′-F′ represent 100 µm and in A-B are 500 µm.