FIGURE

Fig. 2

ID

ZDB-FIG-151106-29

Publication

Ningappa et al., 2015 - The Role of ARF6 in Biliary Atresia

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Fig. 2

arf6 knockdown results in developmental biliary defects in zebrafish.

(A, B) Whole-mount in situ hybridization showing arf6a and arf6b expression in developing embryos/larvae. Arrows point to the liver; arrowheads the pancreas; brackets mark the intestinal bulb. (C) The Tg(Tp1:GFP) and Tg(fabp10a:dsRed) lines reveal the intrahepatic biliary structure and liver size, respectively. Epifluorescence images showing the expression of these transgenes revealed a defect in the intrahepatic biliary structure in arf6-ATG MO-injected larvae. Based on the severity of the biliary defect, larvae were divided into three groups: normal, intermediate, and severe. Graph shows the percentage of larvae in each group. Arrows point to the liver; dotted lines outline the liver (A-C). (D) Epifluorescence images showing PED-6 accumulation in the gallbladder (arrows). Based on PED-6 levels in the gallbladder, larvae were divided into three groups: absent, small/faint, and normal. Graph shows the percentage of larvae in each group. n, the number of larvae examined; scale bars, 100 µm.

Expression Data

Genes:	arf6a arf6b DsRed EGFP
Fish:	WT gz15Tg; um14Tg gz15Tg; um14Tg + MO1-arf6a,arf6b
Knockdown Reagent:	MO1-arf6a,arf6b
Anatomical Terms:	intestinal bulb intrahepatic bile duct liver pancreas
Stage Range:	Protruding-mouth to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gz15Tg; um14Tg + MO1-arf6a,arf6b
Knockdown Reagent:	MO1-arf6a,arf6b
Observed In:	hepatocyte intrahepatic bile duct liver
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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