FIGURE

Fig. S6

ID

ZDB-FIG-151029-32

Publication

Chen et al., 2015 - Transient laminin beta 1a Induction Defines the Wound Epidermis during Zebrafish Fin Regeneration

Other Figures

All Figure Page

Back to All Figure Page

Fig. S6

Tests of association between fgf20a and lamba1.

(A) RT-qPCR analysis for levels of fgf20a in sde1 fin regenerates. qPCR results were normalized to rpl13a and to the basal expression of fgf20a at 0 hpa. (n = 3; mean ± SEM; Student’s t -test, NS, non-significant). (B) Antibody co-staining for Laminin (red) and P63 (green) in longitudinal sections of wild-type and dob fin regenerates at 2 dpa. Scale bars, 50 µm. White arrows indicate where (low) levels of Laminin are detectable. Red arrows indicate plane of amputation. (C) RT-qPCR analysis lamb1a levels in 4 dpa wild-type fin regenerates after 24 hours of treatment with either Igf receptor antagonist NVP-AEW541 (2 µM), or Igf signaling agonist NBI-31772 (10 µM). (D) RT-qPCR analysis of lamb1a levels in 2 dpa sde1/+ and sde1 fin regenerates at the restrictive temperature. qPCR results were normalized to rpl13a and to the basal expression of lamb1a at 0 hpa. (n = 4; mean ± SEM; Student’s t -test, NS, non-significant).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.