FIGURE

Fig. 1

ID

ZDB-FIG-151029-21

Publication

Chen et al., 2015 - Transient laminin beta 1a Induction Defines the Wound Epidermis during Zebrafish Fin Regeneration

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Fig. 1

Forward genetic screen for signaling defects in the fin regeneration epidermis.

(A) Whole-mount images of wild-type and (chc1-9) mutant regenerates at 7 days post amputation (dpa). Red dashed lines indicate plane of amputation. Scale bars, 1 mm. (B) Whole-mount RNA ISH of lef1 expression in wild-type and mutant regenerates at 1 dpa. chc1, chc3, and chc4 mutant families show reduced or undetectable lef1 expression when compared to their respective heterozygous siblings. (C, D) Longitudinal sections of 2 dpa fin regenerates assessed by RNA ISH, showing reduced lef1 and shha in chc1 mutant regenerates. (E) Whole-mount images of sde1 (formerly, chc1) fin regenerates at 4 dpa. Scale bars, 0.5 mm. (F) Longitudinal sections of 3 dpa fin regenerates show impaired patterning of osteoblasts in sde1 (chc1) mutants, assessed by Zns-5 antibody staining (green). DAPI, blue. Scale bars, 50 µm (unless otherwise indicated). Red arrows indicate plane of amputation.

Expression Data

Genes:	lef1 shha
Antibody:	zns-5
Fish:	WT lamb1a^pd110/pd110 lamb1a^pd110/+
Condition:	amputation: caudal fin, heat exposure
Anatomical Terms:	basal regeneration epithelium osteoblast regenerating fin osteoblast regeneration epithelium
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	lamb1a^pd110/pd110
Condition:	amputation: caudal fin, heat exposure
Observed In:	basal regeneration epithelium bone element distal region fin regeneration regenerating fin regenerating fin bone element regenerating fin osteoblast regeneration epithelium
Stage:	Adult

Phenotype Detail

Acknowledgments

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