FIGURE

Fig. 1

ID

ZDB-FIG-151016-25

Publication

Jin et al., 2014 - Prostaglandin signalling regulates ciliogenesis by modulating intraflagellar transport

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Fig. 1

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Expression Data

Genes:	GFP pitx2 spaw
Antibodies:	Ab1-prkcz Ab1-tuba
Fish:	WT abcc4^zf560/zf560 f1Tg abcc4^zf560/zf560; f1Tg
Anatomical Terms:	ependymal cell cilium Kupffer's vesicle epithelial cell Kupffer's vesicle motile cilium lateral crista kinocilium lateral plate mesoderm left side lateral plate mesoderm right side myocardium otic vesicle cilium spinal cord ependymal cell (all 9)
Stage Range:	5-9 somites to Day 4

Expression Detail

Gene	Fish	Conditions	Stage	Anatomy	Assay
GFP	abcc4^zf560/zf560; f1Tg	standard conditions	Long-pec	myocardium	IFL
GFP	f1Tg	standard conditions	Long-pec	myocardium	IFL
pitx2	WT	standard conditions	20-25 somites	lateral plate mesoderm left side	ISH
pitx2	abcc4^zf560/zf560	standard conditions	20-25 somites	lateral plate mesoderm left side	ISH
pitx2	abcc4^zf560/zf560	standard conditions	20-25 somites	lateral plate mesoderm right side	ISH
spaw	WT	standard conditions	20-25 somites	lateral plate mesoderm left side	ISH
spaw	abcc4^zf560/zf560	standard conditions	20-25 somites	lateral plate mesoderm left side	ISH
spaw	abcc4^zf560/zf560	standard conditions	20-25 somites	lateral plate mesoderm right side	ISH

1 - 8 of 8

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Antibody Labeling

Antibody	Fish	Conditions	Stage	Anatomy	Assay
Ab1-prkcz	WT	standard conditions	5-9 somites	Kupffer's vesicle epithelial cell	IHC
Ab1-prkcz	abcc4^zf560/zf560	standard conditions	5-9 somites	Kupffer's vesicle epithelial cell	IHC
Ab1-tuba	WT	standard conditions	5-9 somites	Kupffer's vesicle motile cilium	IHC
Ab1-tuba	WT	standard conditions	Prim-5	otic vesicle cilium	IHC
Ab1-tuba	WT	standard conditions	Long-pec	ependymal cell cilium	IHC
Ab1-tuba	WT	standard conditions	Long-pec	spinal cord ependymal cell	IHC
Ab1-tuba	WT	standard conditions	Day 4	lateral crista kinocilium	IHC
Ab1-tuba	abcc4^zf560/zf560	standard conditions	5-9 somites	Kupffer's vesicle motile cilium	IHC
Ab1-tuba	abcc4^zf560/zf560	standard conditions	Prim-5	otic vesicle cilium	IHC
Ab1-tuba	abcc4^zf560/zf560	standard conditions	Long-pec	ependymal cell cilium	IHC

1 - 10 of 12

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Phenotype Data

Fish:	abcc4^zf560/zf560 abcc4^zf560/zf560; f1Tg
Observed In:	brain brain ventricular system cilium assembly ependymal cell cilium heart heart looping Kupffer's vesicle Kupffer's vesicle motile cilium lateral crista lateral crista kinocilium lateral plate mesoderm lateral plate mesoderm determination of left/right asymmetry in lateral mesoderm otic vesicle otic vesicle cilium otolith post-vent region pronephric duct pronephric duct development spinal cord trunk (all 20)
Stage Range:	5-9 somites to Day 4

Phenotype Detail

Acknowledgments

Full text @ Nat. Cell Biol.