FIGURE

Fig. 3

ID

ZDB-FIG-150624-6

Publication

Wolman et al., 2015 - A Genome-wide Screen Identifies PAPP-AA-Mediated IGFR Signaling as a Novel Regulator of Habituation Learning

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Fig. 3

Acoustic Startle Circuit Appears Intact in pappaa^p170 Larvae

(A-C) DASPEI labeling (A and B) and mean number (C) of sensory neuromasts.

(D-I and K-L) Projections of confocal stacks acquired at 5 dpf. Lateral views, anterior to the left in (A), (B), (D), and (E). Dorsal views, anterior to the top in (F)-(I) and (K)-(L). (D and E) Arrowheads mark projections (anti-neurofilament, red) from cranial ganglia (anti-HuC, green) to hindbrain. (F-I, K, L) Green label marks hspGFF130DMCA:UAS-gap43-citrine expression in Mauthner and Mid2cm Mauthner homologs. (F and G) Open arrows mark Mauthner (M) axon cap. Open arrowhead marks spiral fiber neuron projection and closed arrows mark contralateral passive hyperpolarizing (PHP) projection. (H and I) Brackets mark lateral dendrite of Mauthner with Cx35-positive club endings. Asterisk marks blood cell.

(J) Mean number of club endings.

(K and L) Glycineric receptors on Mauthner soma. SAG, statoacoustic ganglion; aLL, anterior lateral line ganglion; pLL, posterior lateral line ganglion. N, larvae shown within bars. Error bars indicate SEM. Scale bars = 1 mm (B), 50 µm (E), and 10 µm (G, I, and L).

Expression Data

Gene:	Citrine
Antibodies:	Ab-3A10 Ab1-cx35 Ab1-elavl Ab1-glra
Fish:	WT pappaa^p170/p170 nkhspgffdmc131bEt; p201Tg pappaa^p170/p170; nkhspgffdmc131bEt; p201Tg
Condition:	heat shock
Anatomical Terms:	anterior lateral line ganglion anterior lateral line ganglion neuron projection hindbrain interneuron neuron projection Mauthner neuron Mauthner neuron cell body Mauthner neuron dendrite Mauthner neuron synapse MiD2cm posterior lateral line ganglion posterior lateral line ganglion neuron projection statoacoustic (VIII) ganglion statoacoustic (VIII) ganglion neuron projection
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pappaa^p170/p170 pappaa^p170/p170; nkhspgffdmc131bEt; p201Tg
Condition:	heat shock
Observed In:	hindbrain interneuron Mauthner neuron Mauthner neuron synapse neuromast
Stage:	Day 5

Phenotype Detail

Acknowledgments

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