FIGURE

Fig. S8

ID

ZDB-FIG-150618-6

Publication

Marjoram et al., 2015 - Epigenetic control of intestinal barrier function and inflammation in zebrafish

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Fig. S8

A newly identified allele of dnmt1 is similar to uhrf1 and displays elevated TgBAC(tnfa:GFP) expression. (A) cc39.3pd1093 (Middle), a mutant recovered from our forward genetic screen, had a similar phenotype to uhrf1^pd1092 and failed to complement (Top) with dnmt1^s872 (Bottom). (B) Confocal images of crosssections through WT and dnmt1^pd1093 mutants revealed excessive cell shedding (arrows), luminal debris, and a flattened epithelium in mutants. (C and D) Elevated TgBAC(tnfa:GFP) expression was observed in the intestinal epithelium (encircled by a red dotted line) of dnmt1^pd1093 mutants (Upper and Lower Right) but not WT larvae at 120 hpf. Red arrows point to the intestinal epithelium. The yellow frame indicates the plane of section for C and D, Lower. Crosssections (Lower) through WT (C) and dnmt1^pd1093 mutants (D) revealed up-regulation of TgBAC(tnfa:GFP) in the intestinal epithelium of mutants. (E) dnmt1^pd1093 mutants have increased TgBAC(tnfa:GFP) epithelial fluorescence (measured in arbitrary units, a.u.), decreased cell height, and increased cell shedding, similar to uhrf1^pd1092 mutants. ***P < 0.001, ****P < 0.0001.

Expression Data

Gene:	GFP
Fish:	pd1028Tg dnmt1^{pd1093/pd1093}; pd1028Tg
Anatomical Term:	gut epithelium anterior region
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dnmt1^{pd1093/pd1093} dnmt1^{pd1093/pd1093}; pd1028Tg
Observed In:	gut epithelium intestinal epithelial cell
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA