Fig. S8

Fig. S8 A newly identified allele of dnmt1 is similar to uhrf1 and displays elevated TgBAC(tnfa:GFP) expression. (A) cc39.3pd1093 (Middle), a mutant recovered from our forward genetic screen, had a similar phenotype to uhrf1^pd1092 and failed to complement (Top) with dnmt1^s872 (Bottom). (B) Confocal images of crosssections through WT and dnmt1^pd1093 mutants revealed excessive cell shedding (arrows), luminal debris, and a flattened epithelium in mutants. (C and D) Elevated TgBAC(tnfa:GFP) expression was observed in the intestinal epithelium (encircled by a red dotted line) of dnmt1^pd1093 mutants (Upper and Lower Right) but not WT larvae at 120 hpf. Red arrows point to the intestinal epithelium. The yellow frame indicates the plane of section for C and D, Lower. Crosssections (Lower) through WT (C) and dnmt1^pd1093 mutants (D) revealed up-regulation of TgBAC(tnfa:GFP) in the intestinal epithelium of mutants. (E) dnmt1^pd1093 mutants have increased TgBAC(tnfa:GFP) epithelial fluorescence (measured in arbitrary units, a.u.), decreased cell height, and increased cell shedding, similar to uhrf1^pd1092 mutants. ***P < 0.001, ****P < 0.0001.