Fig. S4

Fin regeneration and activation of mTORC1 signaling are inhibited by raptor knock-down. (A,B) Wild-type fin electroporated in the ventral half with fluorescein-labeled MOs at 72 hpa. Fin regeneration was significantly inhibited by raptor knock-down. Dashed lines indicate the amputation planes. Scale bars: 500 µm. **p < 0.01 by Student’s t-test. Error bars represent the standard error of 5 independent experiments. (C) Longitudinal sections of wild-type fin regenerates that were immunostained with antibodies against p-S6K (red) at 24 hpa (5-mismached raptor MO, n = 5; raptor MO, n = 5). Green fluorescence indicates the presence of MO in the electroporated cells. Merged views showed that cells incorporated 5-mismached raptorMO, but not raptor MO, are p-S6K-positive (arrowheads). It should be noted that fluorescent signals of 5- mismached raptorMO and p-S6K overlap only in the cytosol, because p-S6K are localized in the cytosol, not in the nucleus. Dashed white lines indicate the amputation planes. Scale bars: 100 µm.