Fig. 2
- ID
- ZDB-FIG-150514-7
- Publication
- Sonal et al., 2014 - Myosin Vb Mediated Plasma Membrane Homeostasis Regulates Peridermal Cell Size and Maintains Tissue Homeostasis in the Zebrafish Epidermis
- Other Figures
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In absence of Myosin Vb, endosomes and lysosomes accumulate in the cytoplasm of peridermal cells. Time course analysis of vesicle accumulation in control (A1-A4) and myoVb (B1-B4) morpholino injected embryos using Tg(cldnB:lynEGFP) background. Note that the accumulation of vesicles begins at 24hpf. In comparison to wild-type (WT) larva (C) gsp mutant (D) exhibit vesicles in the peridermal cells at 4dpf. Analysis of EGFP-Rab 5 (E,F), EGFP-Rab 11 (G,H), EGFP-Rab7 (I,J) vesicle accumulation at 30 hpf in control morpholino (E,G,I) and myoVb morpholino (F,H,J) injected embryos. Note the increase in EGFP-Rab5, EGFP-Rab11 and EGFP-Rab7 labelled endosomes in morphants (F,H,J). Lamp-1 staining in control (K) and gsp/myoVb mutant (L) embryos using Tg(cldnB:lynEGFP) background reveals increased lysosomal activity in mutants (L). Quantification of Rab5 and Rab11 vesicles in control and myoVb morpholino injected embryos at 30 hpf (M). Arrowheads in E,F indicate EGFP-Rab5 labelled early endosomes; in G,H, EGFP-Rab11 labelled recycling endosomes and in I,J, EGFP-Rab7 labelled late endosomes. Asterisk in ‘M’ indicate that the difference between control and myoVb morpholino injected embryos is statistically significant as per student′s t test (p≤0.05). Scale bars correspond to 20 µ. |
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| Stage Range: | 14-19 somites to Day 4 |
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| Observed In: | |
| Stage Range: | Prim-5 to Day 4 |