ZFIN Image: Sonal et al., 2014, Fig. 2

Image

Figure Caption

Fig. 2

In absence of Myosin Vb, endosomes and lysosomes accumulate in the cytoplasm of peridermal cells.

Time course analysis of vesicle accumulation in control (A1-A4) and myoVb (B1-B4) morpholino injected embryos using Tg(cldnB:lynEGFP) background. Note that the accumulation of vesicles begins at 24hpf. In comparison to wild-type (WT) larva (C) gsp mutant (D) exhibit vesicles in the peridermal cells at 4dpf. Analysis of EGFP-Rab 5 (E,F), EGFP-Rab 11 (G,H), EGFP-Rab7 (I,J) vesicle accumulation at 30 hpf in control morpholino (E,G,I) and myoVb morpholino (F,H,J) injected embryos. Note the increase in EGFP-Rab5, EGFP-Rab11 and EGFP-Rab7 labelled endosomes in morphants (F,H,J). Lamp-1 staining in control (K) and gsp/myoVb mutant (L) embryos using Tg(cldnB:lynEGFP) background reveals increased lysosomal activity in mutants (L). Quantification of Rab5 and Rab11 vesicles in control and myoVb morpholino injected embryos at 30 hpf (M). Arrowheads in E,F indicate EGFP-Rab5 labelled early endosomes; in G,H, EGFP-Rab11 labelled recycling endosomes and in I,J, EGFP-Rab7 labelled late endosomes. Asterisk in ‘M’ indicate that the difference between control and myoVb morpholino injected embryos is statistically significant as per student′s t test (p≤0.05). Scale bars correspond to 20 µ.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.