FIGURE

Fig. 2, S1

ID

ZDB-FIG-150420-30

Publication

Heermann et al., 2015 - Eye morphogenesis driven by epithelial flow into the optic cup facilitated by modulation of bone morphogenetic protein

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Fig. 2, S1

Epithelial flow is independent of cell division.

(A) Retinal cell division was inhibited by application of aphidicolin, a well-established DNA polymerase inhibitor. Aphidicolin efficiently inhibited cell proliferation shown by drastically reduced pHH3 positive nuclei (upper panel, average of 6 pHH3 positive nuclei) compared to the control (lower panel, average of 21 pHH3 positive nuclei) (the optic cup is encircled with a dotted white line, 21.5 hpf). (B) We addressed the epithelial flow of aphidicolin-treated wild-type embryos, injected with H2BGFP RNA at the one cell stage; please see Figure 2A as control. The embryo was preincubated with aphidicolin 5 hr prior to the start of imaging (17 hpf, see also Video 2). The application of aphidicolin did not affect the epithelial flow. As a low level side effect of aphidicolin we observed cell death, in line with previous reports, importantly also not affecting the epithelial flow.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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