FIGURE

Fig. 2

ID
ZDB-FIG-150408-39
Publication
Shiau et al., 2015 - Differential Requirement for irf8 in Formation of Embryonic and Adult Macrophages in Zebrafish
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Fig. 2

irf8 mutant embryos have no macrophages but produce excessive neutrophils.

(A) mfap4 RNA expression at 2 dpf shows a complete loss of macrophages in irf8 mutant but abundant macrophages in heterozygous sibling (arrows). (B) mpx RNA expression at 2 dpf shows an overproduction of neutrophils in irf8 mutants (arrows, bottom panels) compared with the sibling (arrow, top panels). Right column in A and B shows a higher magnification image of the trunk region from the same embryos depicted in the left column. (C) Neutrophils can first be detected on the yolk sac by 1 dpf. irf8 mutants have many yolk sac neutrophils (arrows) compared with sibling. (D) At 2 dpf, irf8 mutants continue to have more neutrophils on the yolk sac (arrows). Overall, irf8 mutants appear to have more neutrophils throughout the body. All scale bars are 100 um.
Expression Data
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage Range: Prim-5 to Long-pec

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One
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