FIGURE

Fig. 2

ID

ZDB-FIG-150406-42

Publication

Sun et al., 2015 - Myc-Induced Liver Tumors in Transgenic Zebrafish Can Regress in tp53 Null Mutation

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Fig. 2

Rapid induction of live tumors by induced mycAG expression.

MycAG fish were induced with 2 µM mifepristone from 1 mpf and sampled at 10, 20 and 30 dpi for gross observation as indicated in the figure. (A1-A4) Gross observation of liver tumors after removal of abdominal wall. (B1-B4) The same views as those in (A1-A4) for observation of GFP expression that illustrate the shape of livers. (C1-C4) H&E staining to show cellular alteration in the fish liver. (D1-D4) PCNA staining by immunocytochemistry to show cell proliferation. (E1-E4) TUNEL assay to reveal the apoptosis in the liver. (F) Quantitative analyses of cell proliferation and apoptosis. indicates highly significant difference (P<0.01) in proliferation when compared that in the Driver control at 30 dpi; § and §§, indicate significant (P<0.05) and highly significant (P<0.01) difference respectively in apoptosis when compared to that in the Driver control at 30 dpi.

Expression Data

Gene:	EGFP
Antibody:	Ab6-pcna
Fish:	gz23Tg gz23Tg; gz27Tg
Condition:	chemical treatment: pharmaceutical
Anatomical Terms:	hepatocyte liver
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gz23Tg; gz27Tg
Condition:	chemical treatment: pharmaceutical
Observed In:	hepatocyte liver liver apoptotic process liver cell population proliferation liver hepatic tumor
Stage:	Adult

Phenotype Detail

Acknowledgments

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