FIGURE

Fig. 2

ID

ZDB-FIG-150406-27

Publication

Boer et al., 2015 - Fascin1-Dependent Filopodia are Required for Directional Migration of a Subset of Neural Crest Cells

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Maternal Fscn1a protein persists throughout embryogenesis and masks morphological defects in fscn1a zygotic mutants.

(A) Brightfield images of 5 dpf wild type (wt), zygotic fscn1a (fscn1a zyg), and maternal/zygotic fscn1a (fscn1a MZ) embryos. Arrow highlights craniofacial cartilage, sb = swim bladder. Number indicates percentage of embryos with phenotype. (B) Immunoblot showing Fscn1a protein levels at 24, 48, 72 hpf and 10 dpf in wt, fscn1a zyg, and fscn1a MZ mutant embryos. Numbers on right denote molecular mass markers. (C) Quantitation of uninjected or fscn1a mRNA-injected fscn1a MZ embryos with normal vs. abnormal craniofacial cartilages at 5 dpf (n = 315 fscn1a MZ embryos, 336 fscn1a MZ + fscn1a mRNA embryos, ****p<0.0001). (D) Quantitation of embryos with normal vs. abnormal craniofacial cartilages at 5 dpf (n = 201 wt female × wt male, 119 fscn1a zyg female × fscn1a zyg male, 115 fscn1a zyg female × wt male, 151 wt female × fscn1a zyg male).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fscn1a^{zd1011/zd1011} fscn1a^{zd1011/zd1011}
Observed In:	heart splanchnocranium swim bladder
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.