PUBLICATION

Fascin1-Dependent Filopodia are Required for Directional Migration of a Subset of Neural Crest Cells

Authors
Boer, E.F., Howell, E.D., Schilling, T.F., Jette, C.A., Stewart, R.A.
ID
ZDB-PUB-150122-3
Date
2015
Source
PLoS Genetics   11: e1004946 (Journal)
Registered Authors
Jette, Cicely A., Schilling, Tom, Stewart, Rodney A.
Keywords
Embryos, Cell migration, Neuron migration, Zebrafish, Neurons, Ganglia, Cartilage, Cancer cell migration
MeSH Terms
  • Animals
  • Body Patterning/genetics
  • Cell Movement/genetics
  • Epithelial-Mesenchymal Transition/genetics
  • Gene Expression Regulation, Developmental
  • Microfilament Proteins/genetics*
  • Mutation
  • Neural Crest/cytology
  • Neural Crest/growth & development*
  • Pseudopodia/genetics*
  • Signal Transduction
  • Zebrafish/genetics
  • Zebrafish/growth & development*
  • Zebrafish Proteins/biosynthesis
  • Zebrafish Proteins/genetics*
(all 15)
PubMed
25607881 Full text @ PLoS Genet.
Abstract
Directional migration of neural crest (NC) cells is essential for patterning the vertebrate embryo, including the craniofacial skeleton. Extensive filopodial protrusions in NC cells are thought to sense chemo-attractive/repulsive signals that provide directionality. To test this hypothesis, we generated null mutations in zebrafish fascin1a (fscn1a), which encodes an actin-bundling protein required for filopodia formation. Homozygous fscn1a zygotic null mutants have normal NC filopodia due to unexpected stability of maternal Fscn1a protein throughout NC development and into juvenile stages. In contrast, maternal/zygotic fscn1a null mutant embryos (fscn1a MZ) have severe loss of NC filopodia. However, only a subset of NC streams display migration defects, associated with selective loss of craniofacial elements and peripheral neurons. We also show that fscn1a-dependent NC migration functions through cxcr4a/cxcl12b chemokine signaling to ensure the fidelity of directional cell migration. These data show that fscn1a-dependent filopodia are required in a subset of NC cells to promote cell migration and NC derivative formation, and that perdurance of long-lived maternal proteins can mask essential zygotic gene functions during NC development.
Genes / Markers
Figures
Figure Gallery (15 images) / 2
Show all Figures
Expression
Phenotype
Fish Conditions Stage Phenotype Figure
fscn1azd1011/zd1011controlDay 5
fscn1azd1011/zd1011controlDay 5
fscn1azd1011/zd1011controlDay 5
fscn1azd1011/zd1011controlDay 5
fscn1azd1011/zd1011standard conditions1-4 somites
fscn1azd1011/zd1011standard conditions10-13 somites
fscn1azd1011/zd1011standard conditions10-13 somites
fscn1azd1011/zd1011standard conditions14-19 somites
fscn1azd1011/zd1011standard conditions14-19 somites
fscn1azd1011/zd1011standard conditionsPrim-5
1 - 10 of 52
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ir937TgTransgenic Insertion
    sb2TgTransgenic Insertion
      vu234TgTransgenic Insertion
        w37TgTransgenic Insertion
          zd1011
            		Small Deletion
            zd1012
              		Small Deletion
              zd1013
                		Indel
                zdf15TgTransgenic Insertion
                  1 - 8 of 8
                  Show
                  Human Disease / Model
                  No data available
                  Sequence Targeting Reagents
                  Target Reagent Reagent Type
                  cxcr4aMO2-cxcr4aMRPHLNO
                  foxd3MO3-foxd3MRPHLNO
                  fscn1aTALEN1-fscn1aTALEN
                  tfap2aMO4-tfap2aMRPHLNO
                  1 - 4 of 4
                  Show
                  Fish
                  Fish
                  fscn1azd1011/zd1011
                  fscn1azd1011/zd1011
                  fscn1azd1011/zd1011 + MO2-cxcr4a
                  fscn1azd1011/zd1011; sb2Tg
                  fscn1azd1011/zd1011; w37Tg
                  fscn1azd1011/zd1011; zdf15Tg
                  fscn1azd1012/zd1012
                  fscn1azd1012/zd1012
                  fscn1azd1012/zd1012; vu234Tg
                  fscn1azd1012/zd1012; vu234Tg + MO2-cxcr4a
                  1 - 10 of 23
                  Show
                  Antibodies
                  No data available
                  Orthology
                  Engineered Foreign Genes
                  Marker Marker Type Name
                  EGFPEFGEGFP
                  GFPEFGGFP
                  mRFPEFGmRFP
                  RFPEFGRFP
                  1 - 4 of 4
                  Show
                  Mapping
                  Your Input Welcome
                  We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
                  Citation
                  Boer, E.F., Howell, E.D., Schilling, T.F., Jette, C.A., Stewart, R.A. (2015) Fascin1-Dependent Filopodia are Required for Directional Migration of a Subset of Neural Crest Cells. PLoS Genetics. 11:e1004946.