Fig. 3
- ID
- ZDB-FIG-150330-19
- Publication
- Friedmann et al., 2015 - A Spinal Opsin Controls Early Neural Activity and Drives a Behavioral Light Response
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Light Inhibition of Coiling Depends on an Extraretinal Opsin (A and B) Representative peristimulus time histograms of coiling frequency (events/s; mean across six trials; bin = 1 s) of embryos at 22–25 hpf (A) or 27–31 hpf (B). Zygotes injected with scrambled control morpholino (n = 23 in A, 34 in B) (black) or morpholino against VALopA (n = 24 in A, 41 in B) (gray). (C) Left, mean photoinhibition (±SEM) of scrambled control morpholino (sc; n = 40) and morpholino (mo; n = 55) -injected 22–25 hpf embryos. Two-tailed unpaired t test, p < 0.001. Right, frequency distribution of percent photoinhibition (six responses per fish) in scrambled control morpholino embryos (black) and morpholino embryos (gray). (D) Normalized inhibition or excitation (mean ± SEM) of coiling frequency [ (HzLight HzDark) / HzDark] in the first 12 s after light onset relative to a 120 s preceding baseline in darkness (n = 39–75). (E) Mean photoinhibition (±SEM; five responses per fish) of morpholino-injected embryos mosaically expressing low, intermediate, or high levels of a VALopA rescue construct with a mCerulean marker. One-way ANOVA with Tukey post hoc analysis, p < 0.05, p < 0.01; n = 59 (low), 18 (intermediate), and 11 (high). (F) Images of representative embryos expressing low, intermediate, and high levels of mCerulean fluorescence. Scale bar, 200 µm. See also Figure S3. |