Fig. 5

Analysis of the eurydendroid-specific GFF line, hspzGFFgDMC156A. (A–G, L–P) The hspzGFFgDMC156A line was crossed with UAS:GFP. Larvae at 5 dpf (A–G) and adult brain sections (L–P) were stained with anti-GFP (green) and anti-Pvalb7 (magenta) antibodies. (A–C) Dorsal views with anterior to the left. (D) Cross-section image of the dotted box in (C). (E) Dorso-anterior view. (F, G) Dorsal (F) and lateral (G) views of eurydendroid cell axons, with anterior to the left. The eurydendroid axons are marked by a dotted line with an arrowhead in F and G. The midlines are indicated by dotted lines in E and F. (L–P) GFP and Pvalb7 expression in the cerebellum of hspzGFFgDMC156A; UAS:GFP adults. (N–P) High magnification views of the box in M. GFP-expressing cells received the axons of Pvalb7⁺ Purkinje cells (marked by asterisks in N–P). (H–K, Q–S) Co-expression of GFP and RFP in hspzGFFgDMC156A; UAS:RFP; olig2:EGFP eurydendroid cells. The 5-dpf larvae (H–K) and adult brain sections (Q–S) were stained with anti-GFP (green) and anti-RFP (magenta) antibodies. (I–K) High magnification views of the box in H. (Q–S) Sagittal sections. Some of the RFP⁺ cells were also GFP⁺ (marked by arrowheads in I–K, Q–S). Projection views (A–H), optical sections (I–K), and histological sections (L–S). Scale bars: 100 µm in A (applied to A–C); 20 µm in D; 50 µm in F; 50 µm in G; 20 µm in H; 20 µm in I (applied to I–K); 200 µm in L (applied to L, M); 50 µm in P (applied to N–P); 50 µm in S (applied to Q–S).