Fig. 9

Detection of cerebellar neural circuitry with WGA tracing. The gSA2AzGFF152B; UAS:GFP line was crossed with UAS:AcGFP-P2A-WGA. Sagittal sections of the adult cerebellum were stained with anti-GFP (green), anti-Pvalb7 (magenta), and anti-WGA (cyan) antibodies. (A-D) and (J-M) represent two different areas of the ML/GCL boundary of the cerebellum. Expression of GFP (A, J), Pvalb7 (B, K), and WGA (C, L), and merged images (D, M) are shown. Projection views of histological sections. (E-I) High magnification views of the box in D. (N-R) High magnification views of the box in M. Optical sections (E-I, N-R). Pvalb7⁺ Purkinje cells that incorporated WGA are indicated by arrowheads (B-D, F-I). Eurydendroid cell with a Pvalb7 soma, that received Pvalb7⁺ axon(s) and incorporated WGA, is indicated by arrowheads (K-M, O-R). Note both Purkinje and eurydendroid cells are GFP-negative. (S, T) Low magnification views of the hindbrain. Expression of GFP (S-V) and WGA (T, V). (U, V) High magnification views of the box in T (IO region). The ventral bottoms of the hindbrain are indicated by dotted lines (U, V). Note that IO neurons incorporated WGA but GFP-negative. Scale bars: 20 µm in A (applied to A-D); 20 µm in E (applied to E-I); 20 µm in J (applied to J-M); 20 µm in N (applied to N-R); 200 µm in S (applied to S, T); 40 µm in U (applied to U, V).