FIGURE

Fig. 8

ID

ZDB-FIG-150318-13

Publication

Gerlach et al., 2014 - Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta

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Fig. 8

Ectopic expression of podocyte genes and early developmental transcription factors in the pronephros is not a general consequence of embryonic defects in fluid flow. WISH was used to assess gene expression using antisense riboprobes to detect wt1a, pax2a, or podxl (purple) in (A) wild-types, (B) prk_CI/ζ morphants, (C) ift88 morphants, (D) tnnt2a morphants, (E) double ift88/tnnt2a morphants, and (F) mpp5a morphants. (A–E) Knockdown of prk_CI/ζ was the only circumstance that led to high levels of ectopic expression of podocyte genes in the pronephros tubule. (F) (Top row) Most mpp5a morphants displayed no ectopic pronephros expression, but (bottom row) a subset (<10%) displayed low levels of ectopic wt1a, pax2a, or podxl in the tubule and also diffuse ectopic expression.

Expression Data

Genes:	pax2a podxl wt1a
Fish:	TU TU + MO1-pals1a TU + MO1-tnnt2a TU + MO2-ift88 TU + MO1-prkci + MO1-prkcz TU + MO1-tnnt2a + MO2-ift88
Knockdown Reagents:	MO1-pals1a MO1-prkci MO1-prkcz MO1-tnnt2a MO2-ift88
Anatomical Terms:	pronephric podocyte pronephric proximal convoluted tubule pronephric proximal straight tubule pronephric tubule
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU + MO1-pals1a TU + MO1-tnnt2a TU + MO2-ift88 TU + MO1-prkci + MO1-prkcz TU + MO1-tnnt2a + MO2-ift88
Knockdown Reagents:	MO1-pals1a MO1-prkci MO1-prkcz MO1-tnnt2a MO2-ift88
Observed In:	establishment or maintenance of epithelial cell apical/basal polarity heart contraction pronephric proximal convoluted tubule cell differentiation involved in pronephros development pronephric proximal straight tubule cell differentiation involved in pronephros development pronephros epithelial cilium movement involved in extracellular fluid movement
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 396(2), Gerlach, G.F., Wingert, R.A., Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta, 183-200, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.