FIGURE

Fig. 8

ID

ZDB-FIG-150219-9

Publication

Thornton et al., 2014 - Selective microRNA uridylation by Zcchc6 (TUT7) and Zcchc11 (TUT4)

Other Figures

Fig. 8

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Fig. 8

Inhibition of zebrafish Zcchc6/11 genes results in improper Hox expression and embryonic development. (a) The zcchc6 or zcchc11 morphant embryos display multiple defects at 48 hpf, including degeneration of somites, failure of tail elongation, pericardial edema and developmental delay. RT-PCR was used to measure the relative knockdown of Zcchc6 and Zcchc11. PCR products were resolved using agarose gel electrophoresis and visualized by ethidium bromide staining (b) Quantitative PCR analysis (on 24-h embryos) shows the reduction of several Hox genes. Blue bars are control and red bars represent Zcchc11 MO (candd) Hoxb8b was reduced in Zcchc6 and Zcchc11 morphant embryos at 24 hpf. The numbers indicated in the right hand corner of each picture: the number of affected embryos similar to what is shown (left) and the total number of observed embryos (right).

Expression Data

Gene:	hoxb8b
Fish:	WT WT + MO1-tut4 WT + MO1-tut7
Knockdown Reagents:	MO1-tut7 MO1-tut4
Anatomical Term:	anatomical structure
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-tut4 WT + MO1-tut7
Knockdown Reagents:	MO1-tut7 MO1-tut4
Observed In:	caudal fin embryo development pericardium somite
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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