FIGURE

Fig. S18

ID

ZDB-FIG-141007-59

Publication

Bielczyk-Maczyńska et al., 2014 - A loss of function screen of identified genome-wide association study Loci reveals new genes controlling hematopoiesis

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Fig. S18

In order to detect macrophages, whole mount in situ hybridization was performed using the probe specific to mpeg1 at 3 dpf. For 13 out of 15 tested MOs (akap10, brd3a, brf1a, brf1b, kalrn1, kalrn2, mfn2, pdia5, psmd13, rcor1, satb1, wasplb and wdr66) there was no observable difference in the number of macrophages. However, knock down of kif1b and waspla resulted in a severe reduction in the number of macrophages in CHT at 3 dpf. All embryos are oriented with anterior to the left and dorsal to the top.

Expression Data

Gene:	mpeg1.1
Fish:	WT WT + MO1-akap10 WT + MO1-brd3a WT + MO1-brf1a WT + MO1-brf1b WT + MO1-cfap251 WT + MO1-kalrna WT + MO1-kalrnb WT + MO1-pdia5 WT + MO1-psmd13 WT + MO1-satb1b WT + MO1-wasla WT + MO1-waslb WT + MO2-mfn2 WT + MO2-rcor1 WT + MO4-kif1b
Knockdown Reagents:	MO1-akap10 MO1-brd3a MO1-brf1a MO1-brf1b MO1-cfap251 MO1-kalrna MO1-kalrnb MO1-pdia5 MO1-psmd13 MO1-satb1b MO1-wasla MO1-waslb MO2-mfn2 MO2-rcor1 MO4-kif1b
Anatomical Term:	macrophage
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-wasla WT + MO4-kif1b
Knockdown Reagents:	MO1-wasla MO4-kif1b
Observed In:	blood island macrophage
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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