FIGURE

Fig. S6

ID

ZDB-FIG-140917-36

Publication

Kanagaraj et al., 2014 - Souffle/Spastizin Controls Secretory Vesicle Maturation during Zebrafish Oogenesis

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Fig. S6

Rescue of chorion elevation defect. (A) Quantification of plasmid injection experiments in Figure 6 H and I showing the number of oocytes with elevated chorions. Error bars represent standard deviation. (B) Morphological phenotype (upper row) of chorion elevation in activated wt (wt + co plasmid) (100%; n = 55), but not in mut oocytes after injection with control DNA (suf + Co plasmid) (0%; n = 56). Mutant oocytes injected with plasmid encoding wt Suf (mut + wt Suf) (87.5%; n = 56) or mut Suf^p96re (mut + mut Suf^p96re) (67.9%; n = 53) show chorion elevation. Rescue of MPA-lectin (middle row) and VAMP4 (lower row) accumulation on immature secretory granules after injection of plasmid encoding wt Suf (mut + wt Suf) or mut Suf^p96re (mut + mut Suf^p96re) into mutant oocytes. Scale bars: 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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