FIGURE

Fig. 2

ID

ZDB-FIG-140915-28

Publication

Law et al., 2014 - The Serine-Threonine Protein Kinase PAK4 Is Dispensable in Zebrafish: Identification of a Morpholino-Generated Pseudophenotype

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Fig. 2

Morphological phenotype of live pak4Δ2/Δ4 mutants.

(A) wild type and F₃ mutant fish at 2 dpf. (B) wild type and F₃ mutant fish at 6 months. Fish were first photographed for documentation followed by fin biopsy genotyping for confirmation. (C) Whole mount in situ hybridization of wild type and mutant embryos at 7-somite for scl. Lateral (left) and dorsal (right) views are shown. Red and blue arrowheads indicate anterior and posterior blood domains, respectively. (D) Whole mount in situ hybridization of wild type and mutant F₃ embryos at 30 hpf for mpo. wt, wild type EK. In panels A, C and D, Δ2/Δ4 refers to the mixed population of homozygous mutant and trans-heterozygous mutant embryos. Panel B shows individuals of each defined genotype, all of which were normal and fertile.

Expression Data

Genes:	mpx tal1
Fish:	WT pak4^y276/+; pak4^y277/+
Anatomical Terms:	granulocyte hematopoietic system
Stage Range:	5-9 somites to Prim-15

Expression Detail

Gene	Fish	Conditions	Stage	Anatomy	Assay
mpx	WT	standard conditions	Prim-15	granulocyte	ISH
mpx	WT	standard conditions	Prim-15	hematopoietic system	ISH
mpx	pak4^y276/+; pak4^y277/+	standard conditions	Prim-15	granulocyte	ISH
mpx	pak4^y276/+; pak4^y277/+	standard conditions	Prim-15	hematopoietic system	ISH
tal1	WT	standard conditions	5-9 somites	hematopoietic system	ISH
tal1	pak4^y276/+; pak4^y277/+	standard conditions	5-9 somites	hematopoietic system	ISH

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Antibody Labeling

No data available

Phenotype Data

Fish:	pak4^y276/+; pak4^y277/+
Observed In:	whole organism
Stage Range:	Long-pec to Adult

Phenotype Detail

Acknowledgments

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