FIGURE

Fig. 5

ID

ZDB-FIG-140903-12

Publication

Heude et al., 2014 - The dlx5a/dlx6a Genes Play Essential Roles in the Early Development of Zebrafish Median Fin and Pectoral Structures

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Knock down of dlx5a/6a leads to a defect of cleithrum differentiation.

Dorsal views of whole mount in situ hybridization for runx2b and col10a1 on control (A, C, E) and dlx5a/6a morphant (B, D, F) embryos at 36 hpf (A–B) and 48 hpf (C–F). In controls at 36 hpf, runx2b is expressed in precursors cells of the cleithrum (cl) (A) whereas expression is absent in the pectoral region of dlx5a/6a morphants (B, black asterisks). At 48 hpf, expression of runx2b and col10a1 is detected in differentiating osteoblasts of the cleithrum which supports the pectoral fin bud (C, E). The runx2b transcripts are also observed at the craniofacial level in the opercular (op) and ceratobranchial 5 (cb5) bone precursors (C). In contrast, dlx5a/6a morphants show a drastic loss of runx2b and col10a1 expression in the pectoral region (D, F, black asterisks) and of runx2b expression at the craniofacial level (D, blue asterisks). Scale bar shown in F for all panels 100 μm.

Expression Data

Genes:	col10a1a runx2b
Fish:	WT WT + MO1-dlx6a + MO4-dlx5a
Knockdown Reagents:	MO1-dlx6a MO4-dlx5a
Anatomical Terms:	ceratobranchial 5 cartilage cleithrum opercle pectoral fin bud cartilage element
Stage Range:	Prim-25 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-dlx6a + MO4-dlx5a
Knockdown Reagents:	MO1-dlx6a MO4-dlx5a
Observed In:	ceratobranchial 5 cartilage cleithrum opercle
Stage Range:	Prim-25 to Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One