Fig. S4

Correlation between lower fluid flow and early left-right markers, Related to Figure 6. These embryos were injected with dnah7-MO to generate a range of weak flow scenarios. (A-C) Embryo with ~ 5 μms^-1 homogeneous flow in the KV, as reported by the flow heat map (A) and the quantification of flow in each KV half (B), this embryo registered strong symmetric charon expression (C). (D-I) as reported by the flow heat maps (D, G) and the quantification of flow in each KV half (E, H), these embryos registered the lowest flows (< 5 μms^-1) and show the weaker symmetric charon expression patterns (F, I). (J-R) Embryos with clearly higher than 5 μms^-1 flow speeds. (J-O) These two embryos have homogeneous flow as reported by the flow heat maps (J, M) and by the quantification of flow in each KV half (K, N), and show strong clear symmetric expression of charon with embryo L slightly stronger than embryo O. (P-R) Injected embryo showing a wild-type pattern of flow as reported by the flow heat map (P) and the quantification of flow in each KV half (Q) showing a weak charon expression on the left side of the KV and strong expression on the right side (R). KV Kupffer’s vesicle; LPM lateral plate mesoderm; N number of particle tracks measured in each embryo; boxplot whiskers represent the minimum and maximum of all of the data.