FIGURE

Fig. S2

ID

ZDB-FIG-140715-74

Publication

Arjona et al., 2014 - CNNM2 Mutations Cause Impaired Brain Development and Seizures in Patients with Hypomagnesemia

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Fig. S2

Impairment of touch-evoked escape behaviour in cnnm2b morphant zebrafish. Touch-evoked escape behaviour score in zebrafish cnnm2b morphants at 5 dpf after injection of 8 ng control-MO/embryo, 8 ng cnnm2b-MO/embryo, 8 ng cnnm2b-MO/embryo+50 pg wild-type (WT) CNNM2 cRNA/embryo, or 2 ng cnnm2b-MO/embryo+50 pg mutant (MT, p.Glu357Lys) CNNM2 cRNA/embryo. Three categories were distinguished, responders, late responders and non-responders, to which the following scores were given: 3 points for responders: fish quickly react (swimming or flicking the tail) to the stimuli after 1 or 2 twitches; 2 points for late responders: fish react (swimming or flicking the tail) to the stimuli after 3, 4 or 5 twitches; and 1 point for non-responders: fish do not react to the stimuli after more than 5 twitches. The upper part of the figure shows frames of videos showing touch-evoked escape contractions at 5 dpf of control and morphant zebrafish larvae. Time of each video frame is indicated in centisenconds (cs). Data are shown as mean ± SEM (n = 30). Different letters indicate significant differences between mean values in experimental groups (P<0.05).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TL + MO1-cnnm2b
Knockdown Reagent:	MO1-cnnm2b
Observed In:	mechanosensory behavior sensory perception of touch
Stage:	Day 5

Phenotype Detail

Acknowledgments

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