FIGURE

Fig. 3

ID

ZDB-FIG-140710-46

Publication

Bu et al., 2014 - Protein tyrosine phosphatase PTPN9 regulates erythroid cell development through STAT3 dephosphorylation in zebrafish

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Fig. 3

ptpn9a knockdown causes no defect in hematopoietic cell proliferation and apoptosis. (A,B) ptpn9a-MOs were injected into Tg(ef1α:mAG-zGem) transgenic embryos, in which green-fluorescence-positive cells are in phases S, G2 or M of the cell cycle. Note that there is little difference in the numbers of green-fluorescence-positive cells in the ICM between ptpn9a-morphant (B) and wild-type (A) embryos at 24 hpf. (C,D) The TUNEL assay showed comparable numbers of apoptotic cells in the ICM in ptpn9a morphants (D) and controls (C) at 24 hpf. (E,F) RNA in situ hybridization revealed that expression of the p53 gene was not induced in the ICM of ptpn9a-morphant embryos (F), compared with wild-type controls (E). Red arrowheads indicate the ICM. The number of phenotypic embryos/total embryos is indicated.

Expression Data

Genes:	mAGFP tp53
Fish:	WT WT + MO1-ptpn9a rw0410hTg rw0410hTg + MO1-ptpn9a
Knockdown Reagent:	MO1-ptpn9a
Anatomical Term:	intermediate cell mass of mesoderm
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.