FIGURE

Fig. S4

ID

ZDB-FIG-140703-14

Publication

Leitch et al., 2014 - Basal body proteins regulate Notch signaling via endosomal trafficking

Other Figures

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Fig. S4

Notch1 localization in ciliated and unciliated cells and suppression of Notch-inhibited cell type markers in bbs4 morphants. (A) hTERT-RPE1 cells doubled immunostained for detection of NOTCH1 (green) and ARL13B (red) and transfected with or without shBBS4. 40X magnification. Cilia in ciliated cells (+) magnified in inset boxes. Scale bars = 20 μm. (B) Expression of neurogenin1 in control MO-, bbs4 MO- and tsg101 MO-injected embryos at 24 hpf detected by whole mount in situ hybridization. (C) Expression of shippo1 in control MO-, bbs4 MO- and tsg101 MO-injected embryos at 24 hpf. Insets indicate magnification of dashed boxes.

Expression Data

Genes:	cimap1b neurog1
Fish:	WT WT + MO1-bbs4 WT + MO1-tsg101a
Knockdown Reagents:	MO1-bbs4 MO1-tsg101a
Anatomical Terms:	central nervous system pronephros
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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