FIGURE

Fig. 4

ID

ZDB-FIG-140430-22

Publication

Wehner et al., 2014 - Wnt/β-Catenin Signaling Defines Organizing Centers that Orchestrate Growth and Differentiation of the Regenerating Zebrafish Caudal Fin

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Fig. 4

Wnt/β-Catenin Signaling in the Proximal Medial Blastema Is Required for Bone Formation

(A) axin1 overexpression in the medial proximal blastema does not significantly reduce H3P⁺ blastemal cells in her4.3:TetA AmCyan; TetRE:Axin1-YFP double transgenic fish. In contrast, proliferation is strongly reduced when axin1 is directed to both the distal and medial proximal blastema using the ubiquitin promoter.

(B) axin1 overexpression in the medial proximal blastema but not in committed osteoblasts reduces the fraction of the regenerate stained with the bone calcification marker Alizarin Red. Bracket indicates the noncalcified distal region of the regenerate.

In (A)-(C), arrowheads, amputation plane. Scale bar, 200 μm. Error bars indicate error of the mean.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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