FIGURE

Fig. 2

ID

ZDB-FIG-140430-10

Publication

Al Oustah et al., 2014 - Dynamics of Sonic hedgehog signaling in the ventral spinal cord are controlled by intrinsic changes in source cells requiring Sulfatase 1

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Fig. 2

Sulf1 depletion impairs generation of ventral neuronal subtypes. Side views of whole embryos at the level of the trunk spinal cord, anterior to the left and dorsal to the top. (A-H) Detection (A-F) and quantification (G,H) of tal2-expressing neurons at 24 hpf in embryos injected with ctrlMO (A), sulf1 mismatch MO (B) and sulf1MO (C) and in wild-type (D), sulf1^sa199+/- (E) and sulf1^sa199-/- (F) embryos. (I-Q) Detection and cell counts of sim1+ (I-K), islet2a+ MNs (L-N) and pax2a+ dorsal neurons (O-Q) in embryos injected with ctrlMO (I,L,O) and sulf1MO (J,M,P). Results are presented as mean number of cells ± s.e.m. (^*P<0.05, ^**P<0.01). Scale bars: 100 μm.

Expression Data

Genes:	isl2a pax2a sim1a tal2
Fish:	WT sulf1^sa199/sa199 WT + MO5-sulf1 sulf1^sa199/+
Knockdown Reagent:	MO5-sulf1
Anatomical Terms:	Kolmer-Agduhr neuron primary motor neuron spinal cord dorsal region spinal cord neuron spinal cord interneuron
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sulf1^sa199/sa199 WT + MO5-sulf1 sulf1^sa199/+
Knockdown Reagent:	MO5-sulf1
Observed In:	Kolmer-Agduhr neuron neuron primary motor neuron spinal cord spinal cord dorsal region spinal cord interneuron ventral spinal cord interneuron differentiation
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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