FIGURE

Fig. 3

ID

ZDB-FIG-140421-3

Publication

Wilkins et al., 2014 - A novel keratin18 promoter that drives reporter gene expression in the intrahepatic and extrahepatic biliary system allows isolation of cell-type specific transcripts from zebrafish liver

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Fig. 3

Tg(krt18:egfp) is expressed at all levels of the developing intrahepatic and extrahepatic biliary system. (A–C) Confocal projections of dissected livers from 3, 4, and 5 dpf Tg(krt18:egfp) fish, indicating biliary proliferation and remodeling (400× magnification). Arrow in panel A indicates rudimentary gallbladder. (D–E) Confocal projections from dissected liver of 5 dpf Tg(krt18:egfp) larva stained with (D) anti-GFP and (E) anti-Mdr to mark biliary cells and hepatocyte canaliculi, respectively. (F) Merged projection from panels D and E, demonstrating colocalization of canaliculi with GFP-positive terminal ductules (D–F: 630× magnification).

Expression Data

Gene:	EGFP
Antibody:	Ab1-mdr
Fish:	p314Tg
Anatomical Terms:	bile canaliculus gall bladder intrahepatic bile duct epithelial cell liver and biliary system
Stage Range:	Protruding-mouth to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Gene expression patterns : GEP, 14(2), Wilkins, B.J., Gong, W., and Pack, M., A novel keratin18 promoter that drives reporter gene expression in the intrahepatic and extrahepatic biliary system allows isolation of cell-type specific transcripts from zebrafish liver, 62-68, Copyright (2014) with permission from Elsevier. Full text @ Gene Expr. Patterns