FIGURE

Fig. 2

ID

ZDB-FIG-140421-2

Publication

Wilkins et al., 2014 - A novel keratin18 promoter that drives reporter gene expression in the intrahepatic and extrahepatic biliary system allows isolation of cell-type specific transcripts from zebrafish liver

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

krt18-driven reporters allow live detection of the developing biliary system. (A) Fluorescent micrograph of live 6 dpf Tg(krt18:egfp) larva, showing EGFP expression in intrahepatic biliary cells (single arrow) and gallbladder epithelium (double arrow), partly obscured by skin expression. (B) and (C) Fluorescent micrographs of live 4 dpf (B) and 7 dpf (C) Tg(krt18:TRAP) larvae, showing visualization of EGFP-Rpl10a fusion protein in developing intrahepatic biliary cells (single arrows) and gallbladder epithelium (double arrow). Red dashed line in (B) indicates gallbladder overlying the liver, out of the focal plane.

Expression Data

Gene:	EGFP
Fish:	p314Tg p315Tg
Anatomical Terms:	gall bladder gall bladder epithelium integument intestine intrahepatic bile duct epithelial cell liver liver and biliary system
Stage Range:	Day 4 to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Gene expression patterns : GEP, 14(2), Wilkins, B.J., Gong, W., and Pack, M., A novel keratin18 promoter that drives reporter gene expression in the intrahepatic and extrahepatic biliary system allows isolation of cell-type specific transcripts from zebrafish liver, 62-68, Copyright (2014) with permission from Elsevier. Full text @ Gene Expr. Patterns