Fig. 5

Reduced expression of EVL-specific genes in Klf17 morphants, but increased expression of mesodermal genes in Klf2b morphants. (A,D) Cellular component and expression pattern annotation of genes downregulated in Klf17 Morpholino KD (A), and genes upregulated in Klf2b KD (D) was performed using GREAT. The charts show the top overrepresented categories belonging to zebrafish expression patterns (blue) and cellular components (red). The x axis values correspond to the binomial raw (uncorrected) P-values. Genes repressed in Klf17 KD show a statistically significant correlation to genes expressed in the extraembryonic EVL, while genes activated in Klf2b KD show a statistically significant correlation to genes expressed in the mesoderm. (B,C) Position-Weight matrices (PWMs) for the motifs overrepresented in the promoters of genes downregulated after Klf17 KD (B) and Klf2a KD (C) match canonical SP1–Klf4 sites. The E-value represents the statistical significance of the motif. (E) Genes normally expressed in the margin are upregulated upon Klf2a, Klf2b individual or double KD. The chart shows fold change values of the microarray probes for aldh1a2, her1, pcdh8, dlc, her7, msgn1, mespa in Klf2a KD, Klf2b KD and double KD compared to control Morpholino injected embryos (values for control Morpholino injected embryos are set to 1). Error bars – standard error of the mean, * – p-value <0.05 in Student′s T-test.