FIGURE

Fig. 4

ID

ZDB-FIG-140324-24

Publication

Samson et al., 2013 - 3-OST-7 regulates BMP-dependent cardiac contraction

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Fig. 4

3-OST-7 controls region-specific BMP signaling in differentiating heart.

ISH for tbx2b (A and D), notch1B (B and E) showed normal AV-restricted expression, whereas bmp4 expression (C and F) showed ectopic expression in ventricular myocardium of 3-OST-7 morphants at 48 hpf (n = 30 for each group). IHC for P-Smad at 48 hpf showed delocalized expression in nuclei of 3-OST-7 morphant ventricle (H) compared to localized AV canal expression in control (G) (n = 10 for each group). (I) Graph depicting increased P-Smad-positive nuclei in the ventricle several unit distances away from the AV in 3-OST-7 morphants compared to P-Smad-positive nuclei localized in the AV for control (error bars, standard deviation). Imaging of live Tg(BRE:d2GFP) fish (J and L) showed GFP expression localized to the AV junction in control (K) and expanded expression in ventricle in morphant (M). V, ventricle; At, atrium; red arrows point to AV; white dashed lines outline the hearts.

Expression Data

Genes:	bmp4 EGFP notch1b tbx2b
Fish:	WT WT + MO1-hs3st1l1 WT + MO2-hs3st1l1 mw30Tg mw30Tg + MO1-hs3st1l1 mw30Tg + MO2-hs3st1l1
Knockdown Reagents:	MO1-hs3st1l1 MO2-hs3st1l1
Anatomical Terms:	atrioventricular canal cardiac ventricle
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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