deltaA expression in trunk neural plate and later neural tube. (A) Dorsal view focused on trunk of a 2- to 3-somite-stage embryo, anterior left, hybridized with digoxygenin-labeled antisense deltaA RNA. High levels of expression are revealed in medial (m) and lateral (l) regions of neural plate. (B) Transverse section through trunk of a 3-somite-stage embryo. Expression is specific to neuroepithelium (between asterisks). (C) Transverse section through trunk of a 20-somite-stage embryo. Cells expressing high levels of deltaA message are largely located near the neural tube basal surface (arrows) but are also in apically positioned cells (asterisk). nc, notochord; s, somitic mesoderm. Scale bar, 40 μm (A), 20 μm (B), 10 μm (C).
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