FIGURE

Fig. 2

ID

ZDB-FIG-140117-18

Publication

Nagashima et al., 2013 - A self-renewing division of zebrafish Muller glial cells generates neuronal progenitors that require N-cadherin to regenerate retinal neurons

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Fig. 2

Müller glia partially dedifferentiate and express retinal progenitor markers in response to photoreceptor lesions. (A-D) PCNA (green), BLBP (cyan) and Rx1 (white/magenta) in control (ctrl) retina. Higher magnifications of the boxed regions are shown on the right. (A) Cones are Rx1⁺ (asterisks, UV cone nuclei). (B) By 1 hpl, Rx1 is reduced in UV cones and BLBP is upregulated in Müller glia. (C) At 8 hpl, cones collapse (asterisk) and Müller glia express BLBP and Rx1 (boxed region). A PCNA⁺ microglia or rod precursor (arrowhead) can be seen in the ONL. (D) At 1 dpl, Müller glia are weakly PCNA⁺/BLBP⁺/Rx1⁺. (E,F) Injury-induced nGFP in lesioned area (asterisks) at 4 and 8 hpl and in immature Müller glia (arrowheads). Zpr1 (magenta), which recognizes Arrestin 3a and specifically labels red/green cones. Higher magnifications of the boxed regions are shown in insets. (C) Reduced rlbp1a transcript (white) by 4 hpl and no detectable signal at 1 dpl. Scale bars: 10 μm (A-D,G); 100 μm (E,F).

Expression Data

Genes:	arr3a EGFP rlbp1a
Antibodies:	Ab1-rx1 Ab3-fabp7 zpr-1
Fish:	WT mi2004Tg
Condition:	light ablation: photoreceptor cell
Anatomical Terms:	Muller cell Muller cell nucleus retinal cone cell
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT mi2004Tg
Condition:	light ablation: photoreceptor cell
Observed In:	eye photoreceptor cell Muller cell retina retina sensory epithelium regeneration
Stage:	Adult

Phenotype Detail

Acknowledgments

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