FIGURE

Fig. 3

ID

ZDB-FIG-140114-27

Publication

Corallo et al., 2013 - Emilin3 is required for notochord sheath integrity and interacts with Scube2 to regulate notochord-derived Hedgehog signals

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Fig. 3

Overexpression of Emilin3 affects notochord patterning activity. (A) Lateral views of 24 hpf embryos probed for ptc1. Embryos were injected with the indicated morpholinos. (B) Wild-type embryos were injected with the indicated constructs at 50 ng/μl and photographed at 24 hpf (upper panels), or probed by in situ hybridization for myoD1 at eight somites and for ehh at 24 hpf (middle panels), or by immunofluorescence for engrailed and Emilin3 at 24 hpf (lower panels). Scale bar: 50 μm. (C) Quantification of engrailed-positive cells in 24 hpf embryos injected as in B (^*P<0.05; n=15). Data are mean+s.e.m. Ctrl MO, control morpholino; e3a+e3b MO, emilin3a + emilin3b morpholinos; MPs, muscle pioneers; MFFs, medial fast fibers.

Expression Data

Gene:	ptch1
Fish:	WT WT + MO2-emilin3a WT + MO2-emilin3b WT + MO2-emilin3a + MO2-emilin3b
Knockdown Reagents:	MO2-emilin3a MO2-emilin3b
Anatomical Terms:	post-vent region trunk
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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