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Fig. 3 Overexpression of Emilin3 affects notochord patterning activity. (A) Lateral views of 24 hpf embryos probed for ptc1. Embryos were injected with the indicated morpholinos. (B) Wild-type embryos were injected with the indicated constructs at 50 ng/μl and photographed at 24 hpf (upper panels), or probed by in situ hybridization for myoD1 at eight somites and for ehh at 24 hpf (middle panels), or by immunofluorescence for engrailed and Emilin3 at 24 hpf (lower panels). Scale bar: 50 μm. (C) Quantification of engrailed-positive cells in 24 hpf embryos injected as in B (*P<0.05; n=15). Data are mean+s.e.m. Ctrl MO, control morpholino; e3a+e3b MO, emilin3a + emilin3b morpholinos; MPs, muscle pioneers; MFFs, medial fast fibers.