FIGURE

Fig. 3

ID

ZDB-FIG-131218-42

Publication

Targoff et al., 2013 - Nkx genes are essential for maintenance of ventricular identity

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Loss of nkx2.7 function in nkx2.5 mutants yields a more prominent ventricular deficiency. (A-F) Frontal views, anterior to the top, of MF20/S46 immunofluorescence, which distinguishes ventricular myocardium (red) from atrial myocardium (yellow), at 52 hpf. In comparison to wild type (A), injection of anti-nkx2.7 MO (D) causes subtle abnormalities in chamber morphology. (B,E) In the nkx2.5 heterozygote, mild atrial enlargement is observed following injection of anti-nkx2.7 MO. (C,F) Injection of anti-nkx2.7 MO into nkx2.5 mutants yields severe loss of ventricular tissue and dramatic expansion of Amhc (S46, yellow) throughout the heart. Arrow indicates ventricular remnant that includes Amhc localization.

Expression Data

Antibodies:	Ab-MF20 Ab-S46
Fish:	WT nkx2.5^vu179/vu179 WT + MO1-nkx2.7 nkx2.5^vu179/+ nkx2.5^vu179/+ + MO1-nkx2.7 nkx2.5^vu179/vu179 + MO1-nkx2.7
Knockdown Reagent:	MO1-nkx2.7
Anatomical Terms:	atrial myocardium ventricular myocardium
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nkx2.5^vu179/vu179 WT + MO1-nkx2.7 nkx2.5^vu179/+ + MO1-nkx2.7 nkx2.5^vu179/vu179 + MO1-nkx2.7
Knockdown Reagent:	MO1-nkx2.7
Observed In:	atrial myocardium heart ventricular myocardium
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development