FIGURE

Fig. 6

ID

ZDB-FIG-131210-50

Publication

Zhen et al., 2013 - Hemogenic endothelium specification and hematopoietic stem cell maintenance employ distinct Scl isoforms

Other Figures

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Fig. 6

scl-a7alpha; is required for the maintenance of HSCs in the AGM. (A-F) cmyb expression in control zebrafish embryos and scl-α morphants. Arrows indicate cmyb expression in AGM (A,B,D,E) or CHT (C,F) region. D, n=43/48; E, n=37/45; F, n=34/40. (G,H) CD41:eGFP⁺ HSCs in the AGM of 2 dpf control embryos and scl-7alpha; morphants (n=25/28). White arrows identify the CD41:eGFP⁺ HSCs and blue arrowheads indicate unaffected CD41:eGFP⁺ cells in the pronephric duct. (I-L2) Time-lapse confocal imaging of a live Tg(scl-α:DsRed; kdrl:eGFP) embryo injected with scl-α MO from <38 to 50 hpf. In J-L′, selected planes show three apoptotic HSCs in the scl-α morphants. Also see supplementary material Movie 4. Similar results were obtained in all four scl-α morphants observed (n=4/4). Scale bars: 50 μm in G,H; 20 μm in I-L2.

Expression Data

Genes:	DsRed EGFP myb
Fish:	WT WT + MO7-tal1 la2Tg la2Tg + MO7-tal1 hkz07tTg; s843Tg + MO7-tal1
Knockdown Reagent:	MO7-tal1
Anatomical Terms:	endothelial cell hematopoietic stem cell nucleate erythrocyte pronephric duct cell ventral wall of dorsal aorta hematopoietic stem cell
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO7-tal1 hkz07tTg; s843Tg + MO7-tal1
Knockdown Reagent:	MO7-tal1
Observed In:	hematopoietic stem cell
Stage Range:	Prim-25 to Protruding-mouth

Phenotype Detail

Acknowledgments

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