FIGURE

Fig. 2

ID

ZDB-FIG-131107-12

Publication

Toyama et al., 2013 - Habenular commissure formation in zebrafish is regulated by the pineal gland-specific gene unc119c

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Fig. 2

Injection of arl3l1 and arl3l2 MO disrupts HC formation. Tg(AANAT2:eGFP) embryos were injected with control (A–C), arl3l1 (D–F, J), or arl3l2 (G–I, K) ATG MO, fixed at 54 hpf and stained with anti-GFP (green, pineal gland) (A, D, and G) and anti-acetylated tubulin (red, neurons) (B, E, and H). C, F, and I are merged images. Arrows indicate the HC (B, E, and H). Dose-dependence of the ATG MO effect (J). K: Splice MOs were injected into wild type embryos, which were stained for acetylated tubulin and classified as above. As the arl3l2 E2I2 MO was somewhat toxic, it was co-injected with 5 ng of p53 MO to suppress cell death (Robu et al., 2007). Experiments were compared to control HC, and statistical significance and number of embryos are indicated as specified in the legend to Figure 1.

Expression Data

Gene:	EGFP
Antibody:	Ab1-tuba
Fish:	y9Tg y9Tg + MO1-arl3l1 y9Tg + MO1-arl3l2
Knockdown Reagents:	MO1-arl3l1 MO1-arl3l2
Anatomical Terms:	caudal commissure epiphysis photoreceptor cell habenular commissure
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	y9Tg + MO1-arl3l1 y9Tg + MO1-arl3l2 y9Tg + MO2-arl3l1 y9Tg + MO2-arl3l2
Knockdown Reagents:	MO1-arl3l1 MO1-arl3l2 MO2-arl3l1 MO2-arl3l2
Observed In:	habenular commissure habenular commissure axon extension involved in axon guidance
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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