FIGURE

Fig. 7

ID

ZDB-FIG-131029-37

Publication

Novikov et al., 2013 - Tmem88a mediates GATA-dependent specification of cardiomyocyte progenitors by restricting WNT signaling

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Fig. 7

Loss of Tmem88a reduces the CP fields. Zebrafish embryos were analyzed at the 8-somite stage. (A,B) WISH for nkx2.5 (box) and ntl in representative control and tmem88a morphants. (C) Relative nkx2.5⁺ areas in control and tmem88a morphants. (D) nkx2.5 relative expression levels as assessed by qPCR. (E,F) WISH for gata4 and ntl in representative control and tmem88a morphants. Arrow indicates the posterior gata4⁺ domain boundary. (G) Relative distance between the notochord tip and the posterior gata4⁺ domain boundary. (H) gata4 relative expression levels by qPCR. (A,B,E,F) Dorsal views of flatmount embryos, with anterior to the top; n>20 embryos analyzed per condition. (C,G) Measurements are normalized to control; n=10 embryos measured per condition. ^**P<0.01; ns, not significant; error bars indicate s.d.

Expression Data

Genes:	gata4 nkx2.5
Fish:	WT WT + MO3-tmem88a + MO4-tmem88a
Knockdown Reagents:	MO3-tmem88a MO4-tmem88a
Anatomical Terms:	anterior lateral plate mesoderm myocardial precursor whole organism
Stage:	5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-tmem88a + MO4-tmem88a
Knockdown Reagents:	MO3-tmem88a MO4-tmem88a
Observed In:	anterior lateral plate mesoderm cardioblast cell fate specification lateral mesoderm morphogenesis myocardial precursor
Stage:	5-9 somites

Phenotype Detail

Acknowledgments

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