FIGURE

Fig. 8

ID

ZDB-FIG-131015-37

Publication

Zhang et al., 2013 - Tissue factor pathway inhibitor-2: A novel gene involved in zebrafish central nervous system development

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Fig. 8

ztfpi-2 morphants exhibited upregulation of huC and ngn1. (A) qPCR analysis showed huC and ngn1 levels have been increased in embryos injected with SP-MO at 25 hpf. ^*P<0.05. (B) WISH showed, at the 3-somite stage, injected embryos had a higher density of neurons in the neural plate than controls. (C) There were more HuC-positive cells in the SP-MO injected HuC-GFP embryos. A z-stack of fluorescence images was acquired by confocal microscopy at 25 hpf. Scale bar: 100 μm. (D) Dorsal views of Mcon-MO and SP-MO injected embryos showing ngn1 expression at 3 somites stage. The changes could be restored by co-injection of SP-MO with ztfpi-2 mRNA.

Expression Data

Genes:	EGFP gfap olig2
Fish:	AB AB + MO1-tfpi2 mi2001Tg mi2001Tg + MO1-tfpi2
Knockdown Reagent:	MO1-tfpi2
Anatomical Terms:	glial cell (sensu Vertebrata) whole organism
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mi2001Tg + MO1-tfpi2
Knockdown Reagent:	MO1-tfpi2
Observed In:	glial cell (sensu Vertebrata)
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 381(1), Zhang, Y., Wang, L., Zhou, W., Wang, H., Zhang, J., Deng, S., Li, W., Li, H., Mao, Z., and Ma, D., Tissue factor pathway inhibitor-2: A novel gene involved in zebrafish central nervous system development, 38-49, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.