FIGURE

Fig. S10

ID

ZDB-FIG-130827-25

Publication

Veldman et al., 2013 - Transdifferentiation of fast skeletal muscle into functional endothelium in vivo by transcription factor etv2

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Fig. S10

Lineage tracing of muscle cells demonstrate they are a source of new blood vessels following Etv2 expression. Lineage tracing of fast muscle fibers in mylpfa:cre-ERt2 injected kdrl:GFP/hsp70l:etv2/ubi:Switch fish demonstrates endothelial cells derived from muscle fibers (arrow). The ubi:Switch transgene changes from GFP to mCherry following recombination initiated by Cre. The mylpfa promoter specifically drives Cre expression in fast muscle fibers. In non–heat shocked embryos (HS) only mCherry muscle fibers are observed (n = 13), while following heat shock mCherry⁺, kdrl:GFP⁺ vessels were observed in 5 out of 15 embryos observed. Note that the kdrl:GFP transgene in the vasculature is significantly brighter than the ubi:Switch GFP⁺ background. All embryos were treated with hydroxytamoxifen (5 μM) immediately after heat shock or the equivalent time for non–heat shocked controls.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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