FIGURE

Fig. S8

ID

ZDB-FIG-130827-23

Publication

Veldman et al., 2013 - Transdifferentiation of fast skeletal muscle into functional endothelium in vivo by transcription factor etv2

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Fig. S8

Fast muscle–specific mylpfa:mRFP is co-expressed with kdrl:GFP following overexpression of Etv2. (A) Confocal image of the trunk of a kdrl:GFP/mylpfa:mRFP/hsp70l:etv2 triple transgenic embryo heat shocked at 24 hpf and imaged at 12 h post–heat shock. A GFP/mRFP double positive muscle fiber is highlighted by the arrow and x-axis and y-axis z-plane projections are presented below and to the right of the image respectively. (B) Fast muscle myosin and GFP colocalize in the trunk of kdrl:GFP/hsp70l:etv2 transgenic fish heat shocked at 24 hpf and imaged at 48 hpf. Red muscle fibers colocalize with GFP (white arrows). A strongly GFP positive cell located where a muscle fiber normally would be is negative for fast muscle myosin (large white arrowhead), suggesting this cell has lost its muscle cell identity.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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